CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
2.3K Views
•
10:40 min
•
April 25th, 2022
DOI :
April 25th, 2022
•Chapters in this video
0:04
Introduction
0:58
Preparation of CRISPR Gene Editing and Guide RNA Design to Generate miRNA Cluster Knockout Cell Lines
2:06
Generation of Stable Lentiviral Cell Lines Carrying the DOX-Inducible Cas9 Expression Cassette
3:20
Performing CRISPR Reactions by Cas9 Induction and Synthetic Guide RNA Transfection of Cells Using a High-Throughput Format
7:22
PCR Genotyping of CRISPR Cell Lines Using Crude Cell Lysates
8:37
Results: High-Throughput CRISPR Experimental Design to Generate Multiple miR-888 Cluster Deletion Combinations
9:51
Conclusion
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