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Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections

13.1K Views

09:23 min

March 26th, 2016

DOI :

10.3791/53976-v

March 26th, 2016


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Double Fluorescence In Situ Hybridization

Chapters in this video

0:05

Title

0:42

Overview of Double Fluoresence In Situ Hybridization Followed by Immunostaining

1:44

RNA Probe Synthesis

4:12

Cryosectioning and Hybridization

5:33

Visualization of the FITC Probe and the DIG Probe

7:23

Immunohistochemistry and Mounting

8:16

Results: Expression of Aspa in Oligodendrocytes in the Cortex and the Corpus Callosum

8:45

Conclusion

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