Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice
2.9K Views
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08:31 min
•
July 20th, 2022
DOI :
July 20th, 2022
•Liza-Anastasia DiCecco*1,2, Samantha Berry*1, G. M. Jonaid*1,3, Maria J. Solares1,4, Liam Kaylor1,4, Jennifer L. Gray5, Carol Bator6, William J. Dearnaley1, Michael Spilman7, Madeline J. Dressel-Dukes8, Kathryn Grandfield2, Sarah M. McDonald Esstman9, Deborah F. Kelly1,5,6
1Department of Biomedical Engineering, Pennsylvania State University, 2Department of Materials Science and Engineering, McMaster University, 3Bioinformatics and Genomics Graduate Program, Huck Institutes of the Life Sciences, Pennsylvania State University, 4Molecular, Cellular, and Integrative Biosciences Graduate Program, Huck Institutes of the Life Sciences, Pennsylvania State University, 5Materials Research Institute, Pennsylvania State University, 6Huck Institutes of the Life Sciences, Pennsylvania State University, 7Applications team, Direct Electron, 8Application Scientist, Protochips, Inc., 9Department of Biology, Wake Forest University
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Chapters in this video
0:05
Introduction
1:43
Loading the Specimen Holder for Liquid-EM
2:47
Production of Microchip Sandwich Assemblies
3:51
Imaging Specimens Using a Transmission Electron Microscope
5:15
Analysis of SARS-CoV-2 Sub-Viral Assemblies in Liquid
6:09
Results: Representative structures of AAV, SARS-CoV-2 Sub-Viral Assemblies and Rotavirus DLPs Prepared in Liquid and Vitreous Ice Using the Microchip Sandwich Technique
7:24
Conclusion
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