JoVE Logo

Sign In

A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline

2.8K Views

09:00 min

June 17th, 2021

DOI :

10.3791/62306-v

June 17th, 2021


Explore More Videos

Sample Preparation

Chapters in this video

0:05

Introduction

0:42

Instrument and Blotting Parameter Set-up

2:53

Crystal Harvest Process

4:01

Light Microscopy for Sample Density Assessment

4:30

Scanning Electron Microscopy for Sample Assessment

5:38

Grid Preparation for the Diffraction Experiments at VMXm

6:47

Results: Analysis of the Microcrystals on the Grids

8:24

Conclusion

Related Videos

article

09:09

Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography

11.4K Views

article

09:35

Microcrystallography of Protein Crystals and In Cellulo Diffraction

9.0K Views

article

08:53

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092

29.8K Views

article

07:11

Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules

6.8K Views

article

07:26

Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers

9.0K Views

article

06:29

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin

5.3K Views

article

09:48

Microcrystal Electron Diffraction of Small Molecules

6.5K Views

article

10:32

Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source

2.6K Views

article

08:35

Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source

4.9K Views

article

07:08

Crystallization and In Situ Room Temperature Data Collection Using the Crystallization Facility at Harwell and Beamline VMXi, Diamond Light Source

3.3K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved