Large-Scale, Automated Production of Adipose-Derived Stem Cell Spheroids for 3D Bioprinting
2.5K Views
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07:40 min
•
March 31st, 2022
DOI :
March 31st, 2022
•Gabriela S. Kronemberger1,2,3, Guilherme A. S. C. Miranda1,2,4, Taisnara I. G. Silva1,2,4, Rosângela M. Gonçalves1,2, José M. Granjeiro2,3,4,5, Leandra S. Baptista1,2,3,4
1Nucleus of Multidisciplinary Research in Biology (Numpex-Bio), Federal University of Rio de Janeiro, 2Laboratory of Tissue Bioengineering, National Institute of Metrology, Quality and Technology (Inmetro), 3Post-graduation Program of Translational Biomedicine (Biotrans), Unigranrio, 4Post-graduation Program in Biotechnology, National Institute of Metrology, Quality and Technology (Inmetro), 5Dental School, Fluminense Federal Fluminense
Chapters in this video
0:05
Introduction
0:55
Trypsinization of ASC Monolayer at Passage Three
1:45
Micro Molded Non-Adherent Agarose Hydrogel Fabrication
2:31
Bio-Fabrication of ASC Spheroids Using the Automated Pipetting System
5:48
Results: Automated Pipette System for Large-Scale Generation of ASC Spheroids
7:17
Conclusion
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