Quantification of Reactive Oxygen Species Using 2′,7′-Dichlorofluorescein Diacetate Probe and Flow-Cytometry in Müller Glial Cells
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14:25 min
•
May 13th, 2022
DOI :
May 13th, 2022
•María V. Vaglienti1,2, Paula V. Subirada1,3, Pablo F. Barcelona1,2, Gustavo Bonacci1,2, María C. Sanchez1,2
1Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, 2Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), 3Instituto de Investigación Médica Mercedes y Martín Ferreyra (INIMEC), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
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Chapters in this video
0:00
Introduction
1:37
Performing the Assay
4:21
Cells Preparaion for Flow Cytometry
6:57
Data Acquisition in a Flow Cytometer
9:00
Analysis of the Acquired Data
12:07
Representative Results
13:37
Conclusion
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