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Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver

4.7K Views

12:07 min

February 18th, 2022

DOI :

10.3791/63333-v

February 18th, 2022


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FAHD1 Protein

Chapters in this video

0:04

Introduction

0:55

Preparation of Buffer, Tissue Sample, and Solution

2:15

Total Protein Extraction

3:42

SDS-PAGE and Western Blot Analysis

4:39

Ammonium Sulfate Precipitation

6:28

Ionic Exchange Chromatography with FPLC

8:14

Protein Extraction Using Defined Sub-Protocols for Ammonium Sulfate-Precipitation and FPLC

9:32

Results: Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver

11:24

Conclusion

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