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Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles

3.7K Views

09:55 min

August 12th, 2021

DOI :

10.3791/62843-v

August 12th, 2021


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Transposon insertion Sequencing

Chapters in this video

0:04

Introduction

0:30

Conjugation to Generate the Transposon Mutant Library

3:32

Mutant Pool Infection on Beetle Eggs

4:43

Sequencing Library Preparation

8:34

Results: Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors

9:11

Conclusion

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