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Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

7.9K Views

08:33 min

August 5th, 2020

DOI :

10.3791/61517-v

August 5th, 2020


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Laser Capture Microdissection

Chapters in this video

0:04

Introduction

1:11

Tissue Fixation

1:47

Tissue Processing

2:38

Paraffin Embedding

3:21

Polyethylene Naphthalate (PEN) Membrane Slide Preparation

3:49

Sectioning

4:53

Laser-Capture Microdissection (LCM)

6:14

Results: Representative LCM Sample Acquisition and RNA Quantification

7:52

Conclusion

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