Accessing the Cytotoxicity and Cell Response to Biomaterials
3.5K Views
•
09:46 min
•
July 8th, 2021
DOI :
July 8th, 2021
•Anabela Baptista Paula*1,2,3,4, Mafalda Laranjo*2,3,4,5, Ana Sofia Coelho1,2,3,4, Ana Margarida Abrantes2,3,4,5, Ana Cristina Gonçalves2,3,4,6, Ana Bela Sarmento-Ribeiro2,3,4,6, Manuel Marques Ferreira2,3,4,7, Maria Filomena Botelho2,3,4,5, Carlos Miguel Marto1,2,3,4,8, Eunice Carrilho1,2,3,4
1Institute of Integrated Clinical Practice, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 2Coimbra Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO), University of Coimbra, Coimbra, Portugal, 3Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, Coimbra, Portugal, 4Clinical Academic Center of Coimbra, CACC, Portugal, 5Institute of Biophysics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 6Laboratory of Oncobiology and Hematology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 7Institute of Endodontics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 8Institute of Experimental Pathology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal
Chapters in this video
0:05
Introduction
1:12
Pellet Preparation and Soluble Biomaterial Extracts Acquisition
2:11
Morphology Evaluation
3:28
Cell Function Assessment
5:54
Mineralized Formation Assessment
6:50
Results: Cell Viability, Morphology, and Protein Expression in Response to Biomaterial Exposure
8:30
Conclusion
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