Flow Cytometry Analysis of Immune Cell Subsets within the Murine Spleen, Bone Marrow, Lymph Nodes and Synovial Tissue in an Osteoarthritis Model
19.3K Views
•
12:23 min
•
April 24th, 2020
DOI :
April 24th, 2020
•Patrick Haubruck1,2, Aimee C. Colbath1,3, Yolanda Liu1, Shihani Stoner1, Cindy Shu1, Christopher B. Little1
1Raymond Purves Bone and Joint Research Laboratory, Institute of Bone and Joint Research, Kolling Institute, Royal North Shore Hospital, University of Sydney, 2HTRG - Heidelberg Trauma Research Group, Center for Orthopedics, Trauma Surgery and Spinal Cord Injury, Trauma and Reconstructive Surgery, Heidelberg University Hospital, 3Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University
Chapters in this video
0:04
Introduction
0:39
Spleen, Contralateral Bone Marrow, Ipsilateral Lymph Nodes, and Synovial Tissue Isolation
4:18
Tissue Processing: Spleen
5:16
Tissue Processing: Lymph Node and Bone Marrow
6:42
Tissue Processing: Synovial Tissue
7:57
Monocyte and T cell Subset Panel Immunostaining
10:28
Results: Representative Mouse Osteoarthritis Model Immune Cell Subset Analysis
11:45
Conclusion
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