Isolation of Stem-like Cells from 3-Dimensional Spheroid Cultures
8.9K Views
•
09:06 min
•
December 13th, 2019
DOI :
December 13th, 2019
•Chapters in this video
0:04
Title
1:16
Culture and Maintenance of HPrEC Cells in 2D
2:30
Labeling HPrEC with BrdU, CFSE, or Far Red Pro-dyes
3:24
Prostasphere Formation in the 3D Basement Membrane Culture System
4:27
Isolation of the CFSE Label-retaining Prostate Stem Cells by FACS Sorting
6:45
Results: Evaluation of the Sphere Based Label-retaining Assay
8:03
Conclusion
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