Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
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09:05 min
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March 11th, 2020
DOI :
March 11th, 2020
•Rafael J. L. Morcillo1, Achen Zhao1,2, María I. Tamayo-Navarrete3, José M. García-Garrido3, Alberto P. Macho1
1Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, 2University of Chinese Academy of Sciences, 3Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín (CSIC)
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Chapters in this video
0:04
Introduction
1:03
Preparation of Tomato Plants and Agrobacterium Rhizogenes
1:55
Plant Transformation and Selection
4:01
Transfer to Inoculation Pots
4:40
Soil-Drenching Inoculation
5:41
Results: Genetic Analysis of Bacterial Wilt Disease
8:20
Conclusion
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