JoVE Logo

Sign In

Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods

7.9K Views

08:37 min

June 25th, 2019

DOI :

10.3791/59308-v

June 25th, 2019


Explore More Videos

Orexin Receptors

Chapters in this video

0:04

Title

1:13

Tissue Cutting

1:45

Blocking of Nonspecific Binding Sites and Tissue Permeabilization

2:44

Incubation with Antibodies

3:18

Tissue Mounting

3:56

Controls

4:26

Image Acquisition

5:31

Results: Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish

7:18

Conclusion

Related Videos

article

08:39

Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos

5.6K Views

article

09:33

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

8.1K Views

article

10:55

Retrograde Labeling of Retinal Ganglion Cells in Adult Zebrafish with Fluorescent Dyes

9.4K Views

article

09:42

Electrophysiological Recording in the Brain of Intact Adult Zebrafish

21.4K Views

article

07:29

Whole Mount Immunohistochemistry in Zebrafish Embryos and Larvae

19.6K Views

article

An Efficient Protocol to Assess ERK Activity Modulation in Early Zebrafish Noonan Syndrome Models via Live FRET Microscopy and Immunofluorescence

198 Views

article

07:36

In Situ Hybridization Combined with Immunohistochemistry in Cryosectioned Zebrafish Embryos

2.7K Views

article

05:12

In Vivo Confocal Fluorescence Imaging of Neural Activity Induced by Sensory Stimulation in Partially Restrained Larval Zebrafish

175 Views

article

05:28

Monitoring On-Target Signaling Responses in Larval Zebrafish - Z-REX Unmasks Precise Mechanisms of Electrophilic Drugs and Metabolites

727 Views

article

00:04

Zebrafish Optogenetics: Activating Genetically Modified Somatosensory Neuron to Study Larval Behavioral Responses

2.0K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved