An Unbiased Approach of Sampling TEM Sections in Neuroscience
7.1K Views
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10:56 min
•
April 13th, 2019
DOI :
April 13th, 2019
•Stefan Wernitznig1, Florian Reichmann2, Mariella Sele1, Christoph Birkl3, Johannes Haybäck4,5, Florian Kleinegger4, Anna Birkl-Töglhofer4, Stefanie Krassnig4, Christina Wodlej4, Peter Holzer2, Daniel Kummer1, Elisabeth Bock1, Gerd Leitinger1
1Department of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center, Medical University of Graz, 2Department of Pharmacology, Otto Loewi Research Center, Medical University of Graz, 3Division of General Neurology, Department of Neurology, Medical University of Graz, 4Institute of Pathology, Medical University of Graz, 5Department of Pathology, Medical Faculty, Otto von Guericke University Magdeburg
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Chapters in this video
0:04
Title
1:02
TEM Preparation Embedding, Ultra-Thin Sectioning and Staining
2:05
Imaging of Corresponding ROIs on the Reference and Look-Up Sections on TEM with Software Packages
5:25
Analyze the TEM images with ImageJ to Document Ultrastructural Features
7:10
Using SerialEM Script to Optimize Elemental Analysis in Brain Samples in Combination with DigitalMicrograph
9:19
Results: Unbiased Approach of Sampling TEM Sections
10:25
Conclusion
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