A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence
8.0K Views
•
13:59 min
•
August 12th, 2018
DOI :
August 12th, 2018
•Chapters in this video
0:00
Title
1:10
Inducing Oncogene-induced Senescence
3:11
Monitoring Senescence by Senescence Associated ß-galactosidase (SA ß-gal) Staining
4:27
Quantifying ROS Induction during OIS
6:42
Quantifying IL-6 and IL-8 mRNA Expression for SASP Anaylsis using Real-time PCR
8:53
Quantifying the Levels of Secreted IL-6 and IL-8 Proteins for SASP Analysis using ELISA
10:56
Results: Representative ROS and SASP Expression Analysis during H-Ras-induced Senescence
12:25
Conclusion
Related Videos
09:18
A Sensitive Method to Quantify Senescent Cancer Cells
20.6K Views
08:18
Induction and Validation of Cellular Senescence in Primary Human Cells
16.6K Views
08:56
Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells
2.8K Views
14:01
Far-Red Fluorescent Senescence-Associated β-Galactosidase Probe for Identification and Enrichment of Senescent Tumor Cells by Flow Cytometry
4.4K Views
08:52
Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry
3.4K Views
14:25
Quantification of Reactive Oxygen Species Using 2′,7′-Dichlorofluorescein Diacetate Probe and Flow-Cytometry in Müller Glial Cells
6.4K Views
11:37
Cellular Redox Profiling Using High-content Microscopy
10.8K Views
06:51
Techniques to Induce and Quantify Cellular Senescence
33.0K Views
07:39
SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs
23.6K Views
09:08
Detecting Reactive Oxygen Species
31.7K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved