Cellular Redox Profiling Using High-content Microscopy
10.8K Views
•
11:37 min
•
May 14th, 2017
DOI :
May 14th, 2017
•1Laboratory of Cell Biology and Histology, Department of Veterinary Sciences, University of Antwerp, 2Cell Systems and Imaging Research Group (CSI), Department of Molecular Biotechnology, Ghent University, 3Department of Biochemistry , Radboud Institute for Molecular Life Sciences, Radboud University Medical Center
Chapters in this video
0:05
Title
0:47
Seeding Cells in a 96-well Plate
1:48
Setting Up the Microscope and Acquisition Protocol
3:10
Loading of the Cells with CM-H2DCFDA and TMRM
4:18
First and Second Live Imaging Rounds
6:22
Image Processing and Analysis
9:35
Results: Effect of Saquinavir on Primary Human Fibroblasts
10:38
Conclusion
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