Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells (NPCs)
9.9K Views
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10:47 min
•
March 2nd, 2018
DOI :
March 2nd, 2018
•Madeline Williams*1, Smrithi Prem*1, Xiaofeng Zhou1, Paul Matteson2, Percy Luk Yeung3, Chi-Wei Lu3, Zhiping Pang4, Linda Brzustowicz5, James H. Millonig2, Emanuel Dicicco-Bloom1
1Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School, 2Center for Advanced Biotechnology and Medicine, Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School, 3The Child Health Institute of NJ, Department of Obstetrics, Gynecology, and Reproductive Services, Rutgers Robert Wood Johnson Medical School, 4The Child Health Institute of NJ, Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School, 5Department of Genetics, Rutgers University
Chapters in this video
0:04
Title
1:08
Collecting NPCs for Plating of Experimental Conditions
2:30
Neural Precursor Cell DNA Synthesis Assay
3:47
S Phase Entry Assay
4:39
Neural Precursor Cell Number Assay
5:41
Neural Precursor Cell Neurosphere Migration Assay
7:58
Results: Use of Phase-contrast and Fluorescence Microscopy to Study the Proliferation, Migration, and Neurite Outgrowth of Neural Precursor Cells
9:51
Conclusion
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