Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types
52.1K Views
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13:05 min
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December 18th, 2014
DOI :
December 18th, 2014
•1Emmy Noether-Group for Stem Cell Biology, Department of Molecular Embryology, Institute of Anatomy and Cell Biology, University of Freiburg, 2Spemann Graduate School of Biology and Medicine and Faculty of Biology, University of Freiburg, 3School of Life Sciences, Keele University, 4Center for Biological Signaling Studies (BIOSS), University of Freiburg
Chapters in this video
0:05
Title
1:03
Neural Cell Harvesting
3:10
Antibody-independent Intracellular Labeling with Carboxyfluorescein Succinimidyl Ester (CFSE)
4:49
Cell-surface Antigen Staining with Fluorophore-conjugated Antibodies
5:56
Fixation and Membrane Permeabilization
7:35
Intracellular Antigen Staining with Non-fluorescent Primary Antibodies
9:30
Flow Cytometry Analysis
10:37
Results: Multicolor Flow Cytometric Analysis of Surface vs. Introcellular Antigens in Neural Cells
12:36
Conclusion
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