Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry
12.9K Views
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11:08 min
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May 5th, 2017
DOI :
May 5th, 2017
•1Flow Cytometry Core Facility, Center for Translational Research-Technical Core, Institut Pasteur, 2Unit for Lymphopoiesis, Immunology Department, INSERM U1223, University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Institut Pasteur, 3Stem-Cell Microenvironments in Repair/Regeneration Team, Instituto de Investigação e Inovação em Saúde (i3s), INEB - Instituto de Engenharia Biomédica, 4ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, 5Stem Cells and Regenerative Medicine Team, UMRS 1166, ICAN - Institute of Cardiometabolism And Nutrition, UPMC - Université Pierre et Marie Curie - Paris 6, INSERM
Chapters in this video
0:05
Title
1:07
Intestinal Cells Isolation
2:20
Cell Staining
3:26
Preparation of Cell Suspension from Embryonic Mouse Heart
5:31
Cardiac Cell Staining
6:54
Data Acquisition and Analysis
8:44
Results: Spectral Cytometry-based Characterization of Embryonic Cardiac Cells
9:50
Conclusion
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