Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
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11.4K Views
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09:25 min
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February 16th, 2017
DOI :
February 16th, 2017
•Mia J. Smith1,2, Thomas A. Packard1, Shannon K. O'Neill1, Rochelle M. Hinman1, Marynette Rihanek3, Peter A. Gottlieb3, John C. Cambier1,4
1Department of Immunology and Microbiology, University of Colorado School of Medicine, 2Department of Microbiology, Immunology, and Pathology, Colorado State University, 3Barbara Davis Center for Childhood Diabetes, University of Colorado School of Medicine, 4Department of Biomedical Research, National Jewish Health
Rozdziały w tym wideo
0:05
Title
1:04
Human Peripheral Blood Mononuclear Cell Isolation (PBMC)
2:19
Cell Staining for Magnetic Nanoparticle-based Enrichment
4:41
Magnetic Nanoparticle-based Enrichment
6:51
Results: Representative Tetanus-toxoid-binding B Cell Post-enrichment Analyses
8:45
Conclusion
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