JoVE Logo

Zaloguj się

Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry

15.2K Views

10:24 min

October 28th, 2014

DOI :

10.3791/51857-v

October 28th, 2014


Przeglądaj więcej filmów

Keywords Cytosolic Calcium

Rozdziały w tym wideo

0:05

Title

1:23

Cell Culture and Calcium Dye Loading

2:36

Preparation of Cells for Flow Cytometry

3:28

Flow Cytometer Setup and Flow Cytometry Measurements

5:33

Data Analysis

7:57

Results: Changes in Cytosolic Calcium Concentration are Measured by Flow Cytometry

9:48

Conclusion

Powiązane Filmy

article

11:37

Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry

11.8K Views

article

11:47

Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells

8.9K Views

article

14:18

Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber

21.1K Views

article

10:01

Analysis of T-cell Receptor-Induced Calcium Influx in Primary Murine T-cells by Full Spectrum Flow Cytometry

3.8K Views

article

12:19

Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli

10.9K Views

article

08:46

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

9.7K Views

article

10:05

Measurement of Calcium Fluctuations Within the Sarcoplasmic Reticulum of Cultured Smooth Muscle Cells Using FRET-based Confocal Imaging

7.6K Views

article

11:32

Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry

13.2K Views

article

12:30

Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+

14.8K Views

article

07:17

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

521 Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone