Detection of Abnormal Prion Protein by Immunohistochemistry
2.8K Views
•
06:38 min
•
May 5th, 2023
DOI :
May 5th, 2023
•Leonor Orge1,2, Maria de Lurdes Pinto1, Paula Cristovão2, Paula Mendonça2, Paulo Carvalho2, Carla Lima3, Helena Santos2, Anabela Alves1, Fernanda Seixas1, Isabel Pires1, Adelina Gama1, Maria dos Anjos Pires1
1Animal and Veterinary Research Centre (CECAV), Associate Laboratory for Animal and Veterinary Science-AL4AnimalS, University of Trás-os-Montes and Alto Douro (UTAD), 2Pathology Laboratory, UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), Oeiras, 3Pathology Laboratory, UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), Vairão
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Chapters in this video
0:04
Introduction
0:48
Deparaffinization and Rehydration
1:39
Epitope Retrieval
2:31
Inactivation of Endogenous Peroxidase and Immunodetection
4:59
Results: Epitope Demasking for Proper PrP Immunolabeling and Minimizing Non-Specific Background Staining
6:00
Conclusion
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