Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis
2.2K Views
•
08:19 min
•
September 28th, 2022
DOI :
September 28th, 2022
•1Key Laboratory of Sustainable Forest Ecosystem Management - Ministry of Education, Northeast Forestry University, 2Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences
Chapters in this video
0:05
Introduction
0:33
Target Design and In Vitro Synthesis of sgRNA
1:18
Embryo Collection and Preparation
2:39
Microinjection of Embryos
4:24
Post-Injection Insect Rearing
5:07
Mutant Screening
6:41
Results: Mutant Screening in B. dorsalis
7:39
Conclusion
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