JoVE Logo

Sign In

Far-Red Fluorescent Senescence-Associated β-Galactosidase Probe for Identification and Enrichment of Senescent Tumor Cells by Flow Cytometry

4.5K Views

14:01 min

September 13th, 2022

DOI :

10.3791/64176-v

September 13th, 2022


Explore More Videos

Flow Cytometry

Chapters in this video

0:04

Introduction

1:16

Induction of Senescence by Chemotherapy Drugs in Cultured Cancer Cells

3:29

DDAO-Galactoside Staining of Senescence-Associated Beta-Galactosidase (SA-β-Gal) in Cell or Tumor Samples

4:56

Flow Cytometer Setup and Data Acquisition

6:27

Flow Cytometry Data Analysis

9:01

Results: Screening Tumors and Chemotherapy Agents for the Induction of Senescence

13:06

Conclusion

Related Videos

article

10:28

Flow Cytometry-based Drug Screening System for the Identification of Small Molecules That Promote Cellular Differentiation of Glioblastoma Stem Cells

8.3K Views

article

10:12

Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization

8.8K Views

article

08:30

Immunophenotyping of Orthotopic Homograft (Syngeneic) of Murine Primary KPC Pancreatic Ductal Adenocarcinoma by Flow Cytometry

12.3K Views

article

08:32

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

9.5K Views

article

05:58

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format

10.8K Views

article

09:18

A Sensitive Method to Quantify Senescent Cancer Cells

20.6K Views

article

08:18

Induction and Validation of Cellular Senescence in Primary Human Cells

16.7K Views

article

08:56

Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells

2.9K Views

article

07:39

SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs

23.7K Views

article

01:26

Flow Cytometry-Based Quantification of Therapy-Induced Senescent Cancer Cells

97 Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved