FACS-Isolation and Culture of Fibro-Adipogenic Progenitors and Muscle Stem Cells from Unperturbed and Injured Mouse Skeletal Muscle
6.0K Views
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11:25 min
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June 8th, 2022
DOI :
June 8th, 2022
•1Laboratory of Muscle Stem Cells and Gene Regulation, National Institute of Arthritis, Musculoskeletal, and Skin Diseases (NIAMS), National Institutes of Health (NIH), 2Flow Cytometry Section, National Institute of Arthritis, Musculoskeletal, and Skin Diseases (NIAMS), National Institutes of Health (NIH)
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Chapters in this video
0:04
Introduction
1:04
Hind Limb Muscle Harvesting
3:11
Mechanical and Enzymatic Muscle Digestion
5:04
Generation of Mononucleated Cells
7:03
Antibody Staining for Flow Cytometry
8:49
Results: FACS-Assisted Simultaneous Isolation and Culture of FAPs and MuSCs from Mouse Limb Muscles
10:37
Conclusion
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