Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria
3.6K Views
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09:56 min
•
October 13th, 2021
DOI :
October 13th, 2021
•Dionysios C. Watson1,2,3, Sadie Johnson1, Akeem Santos1,4, Mei Yin5, Defne Bayik1, Justin D. Lathia1,3, Mohammed Dwidar1,3,4
1Department of Cardiovascular & Metabolic Sciences, Lerner Research Institute, Cleveland Clinic, 2University Hospitals Cleveland Medical Center, 3Case Western Reserve University, 4Center for Microbiome & Human Health, Cleveland Clinic, 5Electron Microscopy Core, Lerner Research Institute, Cleveland Clinic
Chapters in this video
0:05
Introduction
1:09
Culturing Conditions and Extracellular Vesicles Isolation
5:15
Extracellular Vesicles (EVs) Isolation with Size Exclusion Chromatography (SEC)
7:15
EV Preparation Quality Control and Storage
7:58
Results: Analysis of EVs Fraction Collected from Escherichia coli MP1
9:24
Conclusion
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