Longitudinal Intravital Imaging Through Clear Silicone Windows
3.4K Views
•
07:44 min
•
January 5th, 2022
DOI :
January 5th, 2022
•Laura Maiorino*1,2,3, Margaret Shevik*1,4,5, José M. Adrover1, Xiao Han1,6, Elias Georgas7,8, John Erby Wilkinson9, Harrison Seidner1, Leonie Foerschner1, David A. Tuveson1, Yi-Xian Qin8, Mikala Egeblad1
1Cold Spring Harbor Laboratory, 2Cold Spring Harbor Laboratory School of Biological Sciences, 3Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 4Medical Scientist Training Program, School of Medicine, Stony Brook University, 5Graduate Program in Pharmacology, Stony Brook University, 6Graduate Program in Genetics, Stony Brook University, 7Graduate Program in Biomedical Engineering, Stony Brook University, 8Department of Biomedical Engineering, Stony Brook University, 9Department of Pathology, University of Michigan
Chapters in this video
0:04
Introduction
0:50
Casting the Silicone Window and Preparing the Mouse for Insertion of the Silicone Window
1:44
Inserting a Lateral Window for Imaging in the Liver and the Pancreas
3:51
Inserting the Window for Imaging in the Mammary Gland
4:56
Imaging Through the Window
6:04
Results: Investigation of the Adaptability of the PDMS Window to Diverse Tissue Sites
7:05
Conclusion
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