Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles ("Nanoblades")
4.8K Views
•
09:23 min
•
March 31st, 2021
DOI :
March 31st, 2021
•1LBMC, Laboratoire de Biologie et Modélisation de la Cellule Univ Lyon, ENS de Lyon, Université Claude Bernard Lyon 1, CNRS, UMR 5239, INSERM, U1210, Lyon, 69007, France, 2CIRI, Centre International de Recherche en Infectiologie Univ Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, F-69007, Lyon, France
Chapters in this video
0:05
Introduction
0:56
Nanoblade Preparation
2:45
Concentration of Nanoblades on a Sucrose-Cushion
4:16
Monitoring Cas9 Loading Within Nanoblades and Transduction of Cells
6:24
Results: Characterization of Nanoblades
8:29
Conclusion
Related Videos
10:42
A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells
15.1K Views
08:27
Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9
13.4K Views
09:51
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
33.7K Views
13:47
Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
9.5K Views
Enforced Activation of Enhancer RNAs In Situ through the dCas9 Synergistic Activation Mediator System
2.4K Views
04:51
High-Efficiency Gene Disruption in Primary Bone Marrow-Derived Macrophages Using Electroporated Cas9-sgRNA Complexes
1.5K Views
11:28
Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection
17.5K Views
07:20
Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins
12.4K Views
08:14
Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models
12.1K Views
11:32
Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines
10.4K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved