A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells
2.2K Views
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08:01 min
•
August 29th, 2020
DOI :
August 29th, 2020
•1Laboratory of Biomedical Embryology, Department of Health, Animal Science and Food Safety and Center for Stem Cell Research, Università degli Studi di Milano, 2Department of Veterinary Medicine, University of Sassari, 3Department of Agricultural and Environmental Sciences - Production, Landscape, Agroenergy and Center for Stem Cell Research, Università degli Studi di Milano
Chapters in this video
0:04
Introduction
1:11
Fibroblast Exposure to 5-aza-CR
2:28
Fibroblast Encapsulation in PTFE Micro-Bioreactors
4:05
Culture in PTFE Micro-Bioreactors of Epigenetically Erased Cells
5:00
Results: Epigenetically Erased Cells Encapsulated in PTFE Micro-Bioreactors Show High Level and Long-term Maintenance of Pluripotency-Related Gene Expression
7:33
Conclusion
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