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Analyzing the α-Actinin Network in Human iPSC-Derived Cardiomyocytes Using Single Molecule Localization Microscopy

6.6K Views

07:02 min

November 3rd, 2020

DOI :

10.3791/61605-v

November 3rd, 2020


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Actinin Network

Chapters in this video

0:04

Introduction

0:27

Photoactivated Localization Microscopy (PALM) Image Acquisition Setup

1:27

PALM Image Acquisition

1:50

PALM Data Reconstruction

2:39

Reconstructed PALM Image Post Processing

3:34

Sarcomere Filament Analysis

4:41

Results: Representative Human Induced Pluripotent Stem Cell (iPSC)-Derived Cardiomyocyte alpha-Actinin Network Analysis

6:23

Conclusion

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