Manipulation of Single Neural Stem Cells and Neurons in Brain Slices using Robotic Microinjection
4.6K Views
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11:40 min
•
January 21st, 2021
DOI :
January 21st, 2021
•Gabriella Shull*1,2, Christiane Haffner*3, Wieland B. Huttner3, Elena Taverna3,4, Suhasa B. Kodandaramaiah1,5,6
1Department of Biomedical Engineering, University of Minnesota, 2Department of Biomedical Engineering, Duke University, 3Max Planck Institute of Molecular Cell Biology and Genetics, 4Max Planck Institute for Evolutionary Anthropology, 5Department of Mechanical Engineering, University of Minnesota, 6Graduate Program in Neuroscience, University of Minnesota
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Chapters in this video
0:05
Introduction
1:02
Tissue Slice Preparation
4:04
Microinjection
7:05
Tissue Culture and Tissue Slice Processing for Immunofluorescence
9:39
Results: Robotic Microinjection into Apical Progenitors and Neurons
10:57
Conclusion
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