An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants
27.1K Views
•
07:44 min
•
May 22nd, 2020
DOI :
May 22nd, 2020
•Hannes Steinkellner1, Alexander V. Beribisky1, Philip Mausberg1, John Christodoulou2, Barbara Scheiber-Mojdehkar3, Anna Huber1, Victoria Sarne1, Franco Laccone1
1Institute of Medical Genetics, Center for Pathobiochemistry and Genetics, Medical University of Vienna (MUV), 2Murdoch Children's Research Institute and Department of Paediatrics, University of Melbourne, Discipline of Child & Adolescent Health, Sydney Medical School, 3Department of Medical Chemistry and Pathobiochemistry, Medical University of Vienna
Chapters in this video
0:04
Introduction
0:35
Preparing Solutions and Plates (Day 1)
1:50
Blocking and Sample Addition (Day 2)
3:42
Antibody Addition
5:17
Reading the Plate
6:10
Results: Accuracy and Consistency of MeCP2-ECLIA
7:20
Conclusion
Related Videos
09:58
An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
15.6K Views
11:15
Pooled shRNA Screen for Reactivation of MeCP2 on the Inactive X Chromosome
7.2K Views
10:33
Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase
8.1K Views
Non-Radioactive In Vitro Cardiac Myosin Light Chain Kinase Assays
1.8K Views
06:07
Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
2.5K Views
10:15
Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
43.4K Views
09:15
Electrochemiluminescence Assays for Human Islet Autoantibodies
15.4K Views
11:03
Use of Capillary Electrophoresis Immunoassay to Search for Potential Biomarkers of Amyotrophic Lateral Sclerosis in Human Platelets
7.2K Views
10:12
A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay
16.6K Views
02:10
Electrochemiluminescence Assay for Quantification of Target Protein Levels in Brain Lysate
218 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved