Simultaneous Quantification of Selected Kynurenines Analyzed by Liquid Chromatography-Mass Spectrometry in Medium Collected from Cancer Cell Cultures

0:57

3-Nitrotyrosine (3NT), Kynurenine (Kyn), 3-Hydroxykynurenine (3HKyn), 3-Hydroxyanthranilic Acid (3HAA), Xanthurenic Acid (XA) and Charcoal Treated Culture Medium Preparation

1:58

Calibration Solution Preparation

3:42

Liquid Chromatography-Mass Spectrometry (LC-MS) System Setu

4:20

Calibration Curve Construction

4:55

Sample Collection

5:33

Sample Preparation for LC-MS Analysis

6:30

Protein Content Assessment and Data Normalization

8:12

Results: Representative LC-MS Data of Selected KYNURENINES Determination

9:57

Conclusion