Parallel Interrogation of β-Arrestin2 Recruitment for Ligand Screening on a GPCR-Wide Scale using PRESTO-Tango Assay

0:43

Primary Screening: Poly-L-Lysine Plate Preparation

1:25

Primary Screening: Cell Culture and Plate Seeding

2:22

Primary Screening: DNA Plate Preparation and Transfections

3:35

Primary Screening: Cell Stimulation and Luminescence Reading

4:26

Secondary Screening: Cell Seeding and Transfections

5:59

Secondary Screening: Half Log Dose-Curve Drug Plate Preparation and Luminescence Reading

7:16

Results: Representative Primary Screening Hit Identification and Secondary Screening Dose-Curve Responses

8:23

Conclusion