Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
6.7K Views
•
11:32 min
•
March 27th, 2020
DOI :
March 27th, 2020
•Chapters in this video
0:04
Introduction
0:39
Lentiviral Vector Library Preparation
1:24
Packaging of the Arrayed Lentiviral Library
3:35
Cell Infection for Screening
4:48
Cell Seeding for Transfection of Dual-Luciferase Reporter
6:27
Dual-Luciferase Reporter Transfection
7:35
Dual-Luciferase Activity Quantification
9:02
Results: Representative Arrayed Library Screening
10:47
Conclusion
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