Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture
8.8K Views
•
10:38 min
•
November 22nd, 2019
DOI :
November 22nd, 2019
•Chapters in this video
0:05
Title
0:52
Plating Sorted Prostate Epithelial Cells into Primary Mouse Organoid Culture
3:29
Extracting Protein Lysate from Prostate Organoids for Western Blot Analysis
4:50
Fixing and Staining Prostate Organoids for Immunohistochemical Analysis by Whole-Mount Confocal Microscopy
6:44
Tissue Clearing and Mounting of the Stained Prostate Organoids for Whole-mount Confocal Microscopy
7:57
Results: Analysis of Lineage Marker Expression in Prostate Organoids by Western Blot and Whole-Mount Confocal Microscopy
9:44
Conclusion
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