Isolation of Exosome-Enriched Extracellular Vesicles Carrying Granulocyte-Macrophage Colony-Stimulating Factor from Embryonic Stem Cells
4.1K Views
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12:02 min
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November 11th, 2021
DOI :
November 11th, 2021
•Shuhan Meng1,2, Aaron G. Whitt1,2,3, Allison Tu2, John W. Eaton1,2,3, Chi Li1,2,3, Kavitha Yaddanapudi4,5,6
1Department of Pharmacology and Toxicology, University of Louisville, 2Experimental Therapeutics Program, Brown Cancer Center, University of Louisville, 3Department of Medicine, University of Louisville, 4Department of Surgery, University of Louisville, 5Immuno-Oncology Program, Brown Cancer Center, University of Louisville, 6Department of Microbiology and Immunology, University of Louisville
Chapters in this video
0:04
Introduction
1:01
ES-D3 Cell Culture
3:06
Exosome-Enriched Extracellular Vesicle (EV) Isolation
4:09
Exosome-Enriched EV Transmission Electron Microscopy (TEM) Characterization
5:12
Whole Cell Extract (WCE) and EV Lysate Preparation
6:29
Western Blot Analysis
7:11
Enzyme-Linked Immunosorbent Assay (ELISA)
8:48
Results: Representative GM-CSF Expression Analyses
11:13
Conclusion
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