Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii
12.8K Views
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12:22 min
•
August 18th, 2019
DOI :
August 18th, 2019
•Titis A.K. Wardhani1,2, Yuda Purwana Roswanjaya1,2, Simon Dupin1,3, Huchen Li1,4, Sidney Linders1, Marijke Hartog1, Rene Geurts1, Arjan van Zeijl1
1Laboratory of Molecular Biology, Department of Plant Sciences, Wageningen University & Research, 2Center of Technology for Agricultural Production, Agency for the Assessment and Application of Technology (BPPT), 3Department of Ecological Science, Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, 4Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture
Chapters in this video
0:04
Title
1:08
Growth of P. andersonii Trees
3:14
Stable transformation of P. andersonii
6:40
Preparation of Rooted P. andersonii Plantlets
7:31
Nodulation of P. andersonii Plantlets in Pots
9:48
Results: Stable Transformation Procedure
11:13
Conclusion
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