A Stainless Protocol for High Quality RNA Isolation from Laser Capture Microdissected Purkinje Cells in the Human Post-Mortem Cerebellum
7.4K Views
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09:04 min
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January 17th, 2019
DOI :
January 17th, 2019
•1Department of Pathology and Cell Biology, Columbia University, 2Division of Movement Disorders, Department of Neurology, Yale University, 3Department of Chronic Disease Epidemiology, Yale School of Public Health, Yale University, 4Center for Neuroepidemiology and Clinical Neurological Research, Yale School of Medicine, Yale University
Chapters in this video
0:04
Title
0:33
Prepare Cryostat for Tissue Sectioning
1:51
Sectioning Tissue
3:50
Fixing Tissue/Stain-less Visualization
4:47
Laser Capture Microdissection of Purkinje Cells
6:09
Results: RNA Isolation from Laser Capture Microdissected Purkinje Cells
7:59
Conclusion
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Laser Capture Microdissection to Isolate Purkinje Cells from a Human Post-Mortem Cerebellum Tissue
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