In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (THGM) Cells
7.2K Views
•
10:27 min
•
September 10th, 2018
DOI :
September 10th, 2018
•Chapters in this video
0:04
Title
1:01
Murine Splenocyte Preparation
2:10
CD4+ T Cell Magnetic Bead Separation
3:48
Naïve (CD25-CD44loCD62Lhi) CD4+ T Cell Fluorescence-activated Cell Sorting (FACS) and Granulocyte Macrophage-colony-stimulating Factor (GM-CSF) T Helper (THGM) Cell Differentiation
5:48
Mouse THGM Cell Analysis
7:35
Results: THGM Cytokine Profile Analysis
9:30
Conclusion
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