High-sensitivity Detection of Micrometastases Generated by GFP Lentivirus-transduced Organoids Cultured from a Patient-derived Colon Tumor
9.9K Views
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09:16 min
•
June 14th, 2018
DOI :
June 14th, 2018
•Yu Okazawa1,2, Kosuke Mizukoshi1,2, Yu Koyama2,4, Shoki Okubo5, Hiromitsu Komiyama1, Yutaka Kojima1, Michitoshi Goto1, Sonoko Habu3, Okio Hino2, Kazuhiro Sakamoto1, Akira Orimo2
1Department of Coloproctological Surgery, Juntendo University Faculty of Medicine, 2Department of Molecular Pathogenesis, Juntendo University Faculty of Medicine, 3Atopy Research Center, Juntendo University Faculty of Medicine, 4Department of Oral Pathobiological Science and Surgery, Tokyo Dental College, 5Department of Gastroenterology, Juntendo University Faculty of Medicine
Chapters in this video
0:04
Title
0:57
Colorectal Cancer Cell (CRC) Organoid Artificial Extracellular Matrix Culture Installation
2:42
CRC Organic Cell-labeling with GFP Lentiviral Particles
5:28
GFP-labeled CRC Organoid Metastasis Generation
7:55
Results: Representative High-resolution GFP Visualization in Cultured GFP-labeled CRC Organoids, Primary Tumors, and Micrometastases
8:38
Conclusion
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