Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy
9.6K Views
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08:47 min
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December 7th, 2017
DOI :
December 7th, 2017
•1The Ph.D. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, 2Integrated Laboratory, Center of Translational Medicine, Taipei Medical University, 3Core Facility, Taipei Medical University, 4The Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University, 5Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University, 6TMU research center for Neurotrauma and Neuroregeneration, College of Medical Science and Technology, Taipei Medical University
Chapters in this video
0:05
Title
1:00
Primary Neuron Culture and Brain Tissue Isolation
2:56
Tissue Digestion, Plating, and Culture
4:32
Live Image Recording
6:48
Results: The Snapshots of GFP-GLUT4 in Hypothalamic Neurons
7:47
Conclusion
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Imaging GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy
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