Long-term Live Imaging Device for Improved Experimental Manipulation of Zebrafish Larvae
8.7K Views
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14:05 min
•
October 27th, 2017
DOI :
October 27th, 2017
•Kayla Huemer*1,2, Jayne M. Squirrell*3, Robert Swader2, Kirsten Pelkey2, Danny C. LeBert4, Anna Huttenlocher5,6, Kevin W. Eliceiri1,2,3
1Department of Biomedical Engineering, University of Wisconsin-Madison, 2Morgridge Institute for Research, University of Wisconsin-Madison, 3Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison, 4Cellular and Molecular Pathology Graduate Program, University of Wisconsin-Madison, 5Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, 6Department of Pediatrics, University of Wisconsin-Madison
Chapters in this video
0:05
Title
0:57
Design and 3D Printing of Molds
2:57
PDMS Fabrication of zWEDGI Device
4:43
Plasma-bonding zWEDGI to Glass Dish
7:19
Channel Preparation and Loading Larvae
9:52
Wounding and Imaging Larvae and End of Experiment
11:14
Results: Post Wound Live Imaging and 3D Reconstruction of Zebrafish Larvae Tail Fin
12:36
Conclusion
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