Metabolic Glycoengineering of Sialic Acid Using N-acyl-modified Mannosamines
12.6K Views
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12:06 min
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November 25th, 2017
DOI :
November 25th, 2017
•1Max von Pettenkofer-Institut & Genzentrum, Virologie, Nationales Referenzzentrum für Retroviren, Medizinische Fakultät, LMU München, 2Institut für Laboratoriumsmedizin, klinische Chemie und Pathobiochemie, Charité - Universitätsmedizin Berlin, 3Institut für Physiologische Chemie, Martin-Luther-Universität Halle-Wittenberg
Chapters in this video
0:05
Title
1:02
Synthesis of ManNProp and Related N-acyl-modefied Mannosamines
2:26
Cell Culture and Cell Lysis for HPLC Analysis
4:28
Separation of the Membrane Fraction and Acid Hydrolysis
5:27
Fluorescent Labeling, HPLC, and Mass Spetrometry Analysis
6:55
Western Blot Analysis of Polysialic Acid in Kelly Neuroblastoma Cells
9:00
Results: Modulating Expression of Sialic Acid on Cell Surfaces Using N-Side Chain Modified Mannosamines
10:40
Conclusion
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