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High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

10.6K Views

11:24 min

May 13th, 2017

DOI :

10.3791/55623-v

May 13th, 2017


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High Precision FRET

Chapters in this video

0:05

Title

1:07

Preparation of Materials

2:57

Pulsed Interleaved Excitation-Multiparameter Fluorescence Detection (PIE-MFD) Instrument Setup

6:14

Burst Integration Fluorescence Lifetime Analysis of Standards

7:34

dsDNA and Protein Sample Measurements and Analysis

9:28

Results: Inter-dye Distance Determination in Single Molecules

10:54

Conclusion

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