Anatomically Inspired Three-dimensional Micro-tissue Engineered Neural Networks for Nervous System Reconstruction, Modulation, and Modeling
12.7K Views
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10:45 min
•
May 31st, 2017
DOI :
May 31st, 2017
•Laura A. Struzyna*1,2,3, Dayo O. Adewole*1,2,3, Wisberty J. Gordián-Vélez1,2,3, Michael R. Grovola2,3, Justin C. Burrell2,3, Kritika S. Katiyar2,3,4, Dmitriy Petrov2,3, James P. Harris2,3, D. Kacy Cullen2,3
1Department of Bioengineering, School of Engineering and Applied Science, University of Pennsylvania, 2Center for Brain Injury & Repair, Department of Neurosurgery, Perelman School of Medicine, University of Pennsylvania, 3Center for Neurotrauma, Neurodegeneration & Restoration, Michael J. Crescenz Veterans Affairs Medical Center, 4School of Biomedical Engineering, Drexel University
Chapters in this video
0:05
Title
0:58
Micro-column Fabrication Using Capillary Tubes
2:04
Micro-column Trimming and Sterilization
2:48
Preparation of the Pyramidal Micro-well Array
4:46
Formation of Neuronal Aggretates
5:27
ECM Core Fabrication
6:42
Neuronal Aggregate Seeding of Micro-columns
8:14
Results: Observation of Axonal Growth in Micro-TENNs by Phase-contrast Imaging and Cytoarchitecture by Confocal Microscopy
9:51
Conclusion
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