A Rapid Automated Protocol for Muscle Fiber Population Analysis in Rat Muscle Cross Sections Using Myosin Heavy Chain Immunohistochemistry
11.2K Views
•
05:57 min
•
March 28th, 2017
DOI :
March 28th, 2017
•Konstantin D. Bergmeister1,3, Marion Gröger2, Martin Aman1, Anna Willensdorfer1, Krisztina Manzano-Szalai1, Stefan Salminger1, Oskar C. Aszmann1
1CD Laboratory for the Restoration of Extremity Function, Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical University of Vienna, 2Core Facility Imaging, Core Facilities, Medical University Vienna, 3Department of Hand, Plastic and Reconstructive Surgery, Burn Center, BG Trauma Center Ludwigshafen, Plastic and Hand Surgery, University of Heidelberg
Chapters in this video
0:05
Title
0:43
Staining
2:16
Microscopy
3:45
Muscle Fiber Quantification
4:43
Results: Representative Muscle Fiber Analyses of a Rat Muscle Cross Section
5:27
Conclusion
Related Videos
09:39
Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells
15.2K Views
09:17
Minimally Invasive Muscle Embedding (MIME) - A Novel Experimental Technique to Facilitate Donor-Cell-Mediated Myogenesis
7.7K Views
08:36
Semi-automated Analysis of Mouse Skeletal Muscle Morphology and Fiber-type Composition
10.4K Views
08:41
Dissection of Single Skeletal Muscle Fibers for Immunofluorescent and Morphometric Analyses of Whole-Mount Neuromuscular Junctions
8.3K Views
08:42
Visualizing the Morphological Characteristics of Neuromuscular Junction in Rat Medial Gastrocnemius Muscle
3.5K Views
11:50
Fiber Type and Subcellular-Specific Analysis of Lipid Droplet Content in Skeletal Muscle
4.0K Views
08:17
Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses
15.6K Views
09:49
Identification, Isolation, and Characterization of Fibro-Adipogenic Progenitors (FAPs) and Myogenic Progenitors (MPs) in Skeletal Muscle in the Rat
5.8K Views
10:53
Analysis of the Mitochondrial Density and Longitudinal Distribution in Rat Live-Skeletal Muscle Fibers by Confocal Microscopy
3.1K Views
08:12
Intact Short, Intermediate, and Long Skeletal Muscle Fibers Obtained by Enzymatic Dissociation of Six Hindlimb Muscles of Mice: Beyond Flexor Digitorum Brevis
1.6K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved