Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved (Non-model) Organisms
9.2K Views
•
10:41 min
•
May 9th, 2017
DOI :
May 9th, 2017
•Blake L. Joyce1,2, Asher K. Haug-Baltzell3, Jonathan P. Hulvey4, Fiona McCarthy5, Upendra Kumar Devisetty1,6, Eric Lyons1,2,3
1BIO5 Institute, University of Arizona, 2The School of Plant Sciences, University of Arizona, 3Genetics GIDP, University of Arizona, 4Biology Department, University of Massachusetts Amherst, 5School of Animal and Comparative Biomedical Sciences, University of Arizona, 6CyVerse, University of Arizona
Chapters in this video
0:05
Title
1:03
Set Up the Project, Upload Raw Sequencing Reads, and Assess Reads Using FastQC
3:31
Trim and Quality Filter Raw Reads to Yield High Quality Sequence
4:32
De Novo Transcriptome Assembly Using Trinity in Atmosphere
6:42
Pairwise Differential Expression Using DESeq2 in the DE
7:38
Results: FastQC and rnaQUAST Reports
9:24
Conclusion
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