Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus
9.7K Views
•
11:36 min
•
November 20th, 2016
DOI :
November 20th, 2016
•Chapters in this video
0:05
Title
0:58
Preparation of FRT-containing hPSC Line for Transfection
4:44
Transfection by Nucleofection
6:28
Positive and Negative Selection of Cells Undergoing RMCE
7:28
Expansion and Characterization of RMCE Lines
8:31
Results: RMCE in the AAVS1 of hPSC
10:51
Conclusion
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